ࡱ> |~{c ^)bjbj .XObOb*!3z z    8BD| yO  NNNNNNN$KRU^OO43O!!!jN!N!!n@F(XJ0أҷ\hH NIO0yOH_UV _U@XJXJ_U@K!OO yO_Uz B : Project Information PI name/email__________________________________________ Contact Name/email____________________________________ Project Title_____________________________________ Project description or brief abstract (please include details on the service you are requesting): Platform: Luminex______ Kits desired________ RPPA_________ Species: Human ___ Mouse ______ Other (specify)____________ Sample Type: Serum__Tissue___ Cell Line____ Other (specify)______ Estimate date of sample delivery ________________ iLab project registration date (see next page for details)_____________ Please fill the form below: Group #Sample Group NameNumber of samples 1234Total *please expand the table as needed. One Luminex kit is usually able to assay up to38 samples (76 wells in a 96 well format) in duplicate and the rest of the wells are for standards and QC samples. One assay kit has to be used up once open. Due to the kit configuration, we are not able to do 18 samples now, and save the rest for another time. *Recommended sample replicates: Sample size (number of samples per experimental group) for RPPA project depends on the project goal, sample types, and previous data/knowledge. The exact number of samples for the project should be discussed among the investigator, core director, and statistician during the project initiation phase. As a starting point, we recommend the following biological replicates for each sample. The Core routinely spots all protein lysates in triplicates of arrayed slides. These are technical replicates. Sample TypesBackground# of biological replicates Cell lines or cell cultures>=4Mouse tissues/body fluidPure background>=5Mouse tissues/Body FluidMixed background>=8Human samples >=10 (consult statistician)*The reason that 4 replicates are recommended for cell lines is that 3 replicates are absolutely necessary to generate statistically significant data, and the 1 extra allows room for error or outlier, thus increase the possibility of obtaining statistically significant data. Shixia Huang, Ph.D. Director, Proteomics Core Dan L. Duncan Cancer Center Associate Professor, Dept. Molecular & Cellular Biology Alkek Building forBiomedicalResearch, R507 6Ͽ One Baylor Plaza, MS130 Houston, TX 77030 713-798-8722 (Office) 713-798-8935/6740 (lab) 713-798-1370 (Fax) E-mail:  HYPERLINK "mailto:shixiah@bcm.edu" shixiah@bcm.edu Welcome to iLab Solutions at 6Ͽ! BCM is excited to launch a web-based tool to help manage central resources at the institute. We will be rolling out the software in several phases. The Protein Profiling + Quantitative Proteomics Core would like to invite you to register for an iLab account so you can access core services through the new on-line system. Please follow the instructions below to complete the one-time registration process and get started working with the core! If you already have an account with iLab, you do not need to register again. Simply login and click on core facilities link in the left menu to find the new core listed. BCM Users: Register with iLab  HYPERLINK "https://bcm.corefacilities.org/account/login" Click HYPERLINK "https://bcm.corefacilities.org/account/login"   HYPERLINK "https://bcm.corefacilities.org/account/login" here HYPERLINK "https://bcm.corefacilities.org/account/login"   HYPERLINK "https://bcm.corefacilities.org/account/login" to HYPERLINK "https://bcm.corefacilities.org/account/login"   HYPERLINK "https://bcm.corefacilities.org/account/login" login HYPERLINK "https://bcm.corefacilities.org/account/login"   HYPERLINK "https://bcm.corefacilities.org/account/login" and HYPERLINK "https://bcm.corefacilities.org/account/login"   HYPERLINK "https://bcm.corefacilities.org/account/login" register:  HYPERLINK "https://bcm.corefacilities.org/account/login" https://bcm.corefacilities.org/account/login (click BCM user link once on page). Login using your BCM username and password on the BCM single sign on page. Upon successful authentication, you will redirect into the iLab system. Select your lab from the pull-down list, provide your phone number. Submit the completed registration form. Submission will notify your PI of your lab membership request. You will be sent an email with basic instructions on using the system at this time. However, you will not be able to place orders with your new account until your PI has approved your lab membership request. Non-BCM Users: Sign up for iLab Account Complete the account sign-up form here: HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060"   HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" https HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" :// HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" bcm HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" . HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" corefacilities HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" . HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" org HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" / HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" account HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" / HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" signup HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" /43? HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" sc HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" _ HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" id HYPERLINK "https://bcm.corefacilities.org/account/signup/43?sc_id=3060" =3060 You will receive an email with account information and basic instructions to get started using the system once your account has been approved. You can also access the core's webpage directly, which has login and registration links for all users in the upper right corner.  HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" https HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" :// HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" bcm HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" . HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" corefacilities HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" . HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" org HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" / HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" service HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" _ HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" center HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" / HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" show HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" _ HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" external HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" /3060/ HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" BCM HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" _ HYPERLINK "https://bcm.corefacilities.org/service_center/show_external/3060/BCM_Proteomics" Proteomics If you have any questions, concerns, comments or suggestions, please use the 'leave iLab feedback' link in the upper right hand corner once you are logged in. Alternatively, you can email us at  HYPERLINK "mailto:support@ilabsolutions.com" support@ilabsolutions.com. 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