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Research

Cytometry and Cell Sorting Core Instrumentation Training

Master
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The CCSC has 9 analyzers:

  • 2 BD LSRIIs
  • BD LSRFortessa
  • BD Symphony 
  • 2 BD CantoIIs
  • ThermoFisher Acoustic Focusing Attune NxT
  • Cytek Aurora Spectral analyzer
  • Cytek ImageStreamX Mark II


The core also has 5 sorters:

  • 2 five-laser BD FACSAriaIIs
  • Three-laser BD FACSAriaII
  • Four-laser Sony MA900
  • Two-laser Union Biometrica BioSorter

In addition, we offer:

  • Automated Viability Analyzer/Cell Counter (Vi-Cell)
  • AutoMACS Pro Magnetic Column Cell Separator
  • GentleMACS tissue dissociator
  • Three computers at our workstations (3 PCs)

Training is offered on all instruments.

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Training Types

Content

We offer six main types of training (request all training by email):

  • Analyzer Training - $350 - (acquire data on fluorescence expression). Typically 3 days (4-5 hours apiece) on a Monday-Wednesday.
  • Aria Sort Training - $120/hour - (analyze AND deflect a certain population of cells to be collected for downstream applications). Typically 16-20 hours of one on one training. Requests to be trained will be reviewed on a case by case basis.
  • Sony MA900 Sort Training - $120/hour-(analyze the fluorescence expression AND deflect a certain population of cells to be collected downstream applications). Typically 10-12 hours of one on one training.
  • Vi-Cell Training- Complementary ~30 minutes - (cell counter and viability analyzer).
  • AutoMACS Pro Training- Complementary ~1 hour - (magnetic cell separator).
  • GentleMACS Training - Complementary ~10 minutes- (tissue dissociator).